MycoDDR

Rigid pH Control leads to greater retention of viable Mycobacteria

• Smears are easier to interpret

• Culture results are obtained faster

• Less specimens need to be reprocessed

Digestion/Decontamination Reagents for the Recovery of Mycobacterium


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The MycoDDR™ empowers labs to maximize the retention of viable Mycobacteria via rigid pH control throughout the digestion/decontamination process. Maintaining the correct pH with the MycoDDR™ is simple—it is accomplished by means of visual confirmation. When the Neutralization Buffer B is added, the solution will turn clear or colorless when it reaches the ideal pH range. Also, the Resuspension Buffer C enables labs to obtain a clean, uniform pellet with a pH between 6.8-7.2, which ensures the sample will be accepted in the sensitive environment for all rapid automated growth detection instruments.

NaOH/NALC Reagent A

The NaOH/NALC Reagent A uses an integrated blue, color changing pH indicator, which enables labs to maintain rigid pH control throughout the entire digestion/decontamination process. Unlike other commercial reagents, the NaOH/NALC Reagent A is available in varying concentrations, offering a unique solution for every laboratory. It also maintains stability for 72 hours after the NALC is mixed with the NaOH solution.

Neutralization Buffer B

Following the 15 minute decontamination process it is critical to return the sample to a neutral pH to ensure the maximum recovery of viable Mycobacteria. If the solution is not properly neutralized, the pH will remain at a level that will continue to kill Mycobacteria.

A major shortcoming with common digestion/decontamination methods is that the standard neutralizing agent, m/15 Phosphate Buffer, is incapable of reducing a sample’s pH to the optimum level for Mycobacteria survival. Samples neutralized with phosphate buffer will generally remain at a pH of around 10-12, and viable Mycobacteria will continue to be rapidly killed off during the 15-20 minute centrifugation.

The MycoDDR™ Neutralization Buffer B has an extremely high buffering capacity, allowing it to rapidly neutralize solutions to the optimal range for Mycobacteria survival. Proper neutralization of samples is confirmed via a visual color change. When the sample changes from blue to clear, it is at the correct pH. This ensures maximum recovery of viable Mycobacteria, leading to quicker detection in automated equipment, and reduced risk of missing positive patients.

Resuspension Buffer C

In order to obtain optimal results with downstream testing, it is essential to obtain a uniform sample pellet within the appropriate pH range. The MycoDDR™ Resuspension Buffer C enables labs to obtain a clean, uniform pellet at a pH between 6.8-7.2, which ensures the sample will be accepted in the sensitive environment for all rapid automated growth detection instruments.

Ref #Contents/ComponentsDescription
DDR012-2.512 tests worth of reagents:
1 x 60 mL of NaOH 2.5%
1 x 300 mg NALC
12 x 30 mL Neutralization Buffer B
12 x 3 mL Resuspension Buffer C
Reagent Set 2.5%
DDR012-3.012 tests worth of reagents:
1 x 60 mL of NaOH 3.0%
1 x 300 mg NALC
12 x 30 mL Neutralization Buffer B
12 x 3 mL Resuspension Buffer C
Reagent Set 3.0%
TBNN1010-2.510 x 60 mL of NaOH 2.5%
10 x 300 mg of NALC
2.5% NaOH and NALC – Individual Bulk
TBNN1010-3.010 x 60 mL of NaOH 3.0%
10 x 300 mg of NALC
3.0% NaOH and NALC – Individual Bulk
TBP300-55 x 300 mg NALCNALC - Bulk
TBNN11-2.51 x 60 mL of NaOH 2.5%
1 x 300 mg NALC
Sample 2.5% NaOH and NALC
TBNN11-3.01 x 60 mL of NaOH 3.0%
1 x 300 mg NALC
Sample 3.0% NaOH and NALC
TBPN67-6060 x 30 mLNeutralization Buffer B – Individual Bulk
TBPN67-88 x 500 mLNeutralization Buffer B – Bulk
TBRB30-6060 x 3 mLResuspension Buffer C – Individual Bulk

Posters

Package Inserts

MycoDDR Trident – 2.5% NaOH

MycoDDR Trident – 3.0% NaOH